液相色谱-串联质谱技术在临床检验中的应用研究进展

液相色谱-串联质谱（LC-MS/MS）技术具有高灵敏度、高特异性、高分辨率和高效率的优点。近年来随着仪器灵敏度的提高，LC-MS/MS在常规临床检验中显示出极大的潜力，并在疾病早期预防和诊断中发挥着不可替代的作用。本文对LC-MS/MS在新生儿疾病筛查、维生素D检测、内分泌激素检测、肽类和蛋白质定量分析等临床检验方面的研究进展进行综述，并讨论了未来面临的挑战。     

Comparison of yield and metabolites according to the types of hilling materials utilized during Glehnia littoralis sprout vegetable cultivation

Food Science and Biotechnology - Various hilling materials (rice hulls, pine sawdust, and perlite) were compared to produce sprout vegetables using beach silvertop (Glehnia littoralis Fr. Schm. ex...     

影响荔枝酒发酵过程中挥发酸的因素

挥发酸的控制是荔枝酒的生产过程中的重要技术瓶颈,发酵过程易造成荔枝果酒挥     

政策工具视角下城市水系统全周期管理策略研究

我国城市水系统管理职能条块分割、规划协调不足、精细程度不够等现象突出."全周期管理"可以从全要素、全过程、全场景等角度,以系统化思维解决城市水系统综合管理,增强城市水系统韧性和弹性.基于政策工具视角建立了包含战略面、供给面、需求面、环境面和评估面政策的城市水系统全周期管理整体性框架,并从机构统筹、规划引领、智慧监管等方面提出实施机制建议,为实现城市水系统的全要素管控、全流程监督、跨区域协同、分层次治理提供理论依据.     

煤焦油加氢装置能量优化项目研究

通过对煤焦油加氢装置能量进行梯级利用,优化能源的使用状态,保证温位的合理匹配,实现降本增效,同时消除装置运行瓶颈问题,提高装置加工负荷,优化设备运行,进而提高煤焦油加氢装置的市场竞争力.     

高分子材料导热性能影响因素研究进展

介绍了高分子材料导热性能影响因素研究进展，重点阐释了聚合物基体的结构特性（链结构、分子间相互作用、取向、结晶度等）、导热填料（种类、含量、形态、尺寸等）以及制备方法等对高分子材料导热性能的影响。     

伊拉克米桑油田水平井钻井关键技术

针对伊拉克米桑油田盐膏层巨厚、钻井液密度窗口窄、存在高压盐水层,储层段漏塌同层导致复杂时效高等问题,通过对比分析国内外盐膏层和窄压力窗口地层钻井技术,总结了米桑油田钻井面临的主要技术难点,从井身结构优化、钻井提速和复杂预防等方面入手,研究形成了适合米桑油田的钻井关键技术,并进行了现场试验应用,取得了显著的效果.伊拉克米桑油田钻井关键技术对该地区钻井技术方案设计和钻井提速提效具有较强的借鉴和指导作用.     

Adaptation to Endoplasmic Reticulum Stress Enhances Resistance of Oral Cancer Cells to Cisplatin by Up-Regulating Polymerase η and Increasing DNA Repair Efficiency

Endoplasmic reticulum (ER) stress response is an adaptive program to cope with cellular stress that disturbs the function and homeostasis of ER, which commonly occurs during cancer progression to late stage. Late-stage cancers, mostly requiring chemotherapy, often develop treatment resistance. Chemoresistance has been linked to ER stress response; however, most of the evidence has come from studies that correlate the expression of stress markers with poor prognosis or demonstrate proapoptosis by the knockdown of stress-responsive genes. Since ER stress in cancers usually persists and is essentially not induced by genetic manipulations, we used low doses of ER stress inducers at levels that allowed cell adaptation to occur in order to investigate the effect of stress response on chemoresistance. We found that prolonged tolerable ER stress promotes mesenchymal–epithelial transition, slows cell-cycle progression, and delays the S-phase exit. Consequently, cisplatin-induced apoptosis was significantly decreased in stress-adapted cells, implying their acquisition of cisplatin resistance. Molecularly, we found that proliferating cell nuclear antigen (PCNA) ubiquitination and the expression of polymerase η, the main polymerase responsible for translesion synthesis across cisplatin-DNA damage, were up-regulated in ER stress-adaptive cells, and their enhanced cisplatin resistance was abrogated by the knockout of polymerase η. We also found that a fraction of p53 in stress-adapted cells was translocated to the nucleus, and that these cells exhibited a significant decline in the level of cisplatin-DNA damage. Consistently, we showed that the nuclear p53 coincided with strong positivity of glucose-related protein 78 (GRP78) on immunostaining of clinical biopsies, and the cisplatin-based chemotherapy was less effective for patients with high levels of ER stress. Taken together, this study uncovers that adaptation to ER stress enhances DNA repair and damage tolerance, with which stressed cells gain resistance to chemotherapeutics.     

中国智能机械制造评价及发展研究

以中国规模以上工业企业数据库为基础，结合在智能制造主要区域调研的结果，筛选出15145家智能制造企业，经同行评议后，生成322家候选企业名单；再由资深产业专家从“开发式创新”和“探索式创新”两个方面去考察候选企业的能力，得到中国智能制造百强排行榜；随后根据行业分类，得到智能机械制造企业排行榜。在概述了智能机械制造上榜企业的基本情况后，着重分析了前20强企业，并提出了中国智能机械制造发展路径和未来的发展趋势。